Determination of Lapatinib in Bulk and Tablet Dosage Form using Ultraviolet Spectrophotometric and RP-HPLC Analytical Methods
نویسندگان
چکیده
Objectives: The present article involved the development of sensitive and validated Ultraviolet (UV) Spectrophotometric reverse phase liquid chromatographic method for determination lapatinib in bulk pharmaceutical dosage form. Materials Methods: UV is based on measurement absorption at maximum 268 nm using methanol as a solvent. In High Performance Liquid Chromatography (HPLC) method, Waters HPLC with Empower-2 Software PDA Detector was utilised. Acetonitrile phosphate buffer ratio 65:35 (v/v) utilised mobile phase. Results: drug obeyed beer lambert’s law concentration range 2-20 μg/ml regression coefficient 0.999. linearity level 20-200 μg/ml. developed methods have been successfully applied validation study. percentage purity marketed form found 99.68 % 99.82% method. both methods, percent RSD all parameters examined recommended ICH guidelines to be less than 2. highest degradation (19.58%) occurs alkaline stressed environments force analysis by technique. also associated remaining conditions. Conclusion: two were accurate, simple estimating tablet doses.
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ژورنال
عنوان ژورنال: International journal of pharmaceutical investigation
سال: 2021
ISSN: ['2230-9713', '2230-973X']
DOI: https://doi.org/10.5530/ijpi.2021.2.37